( b, c) Summary (mean ± SD value) of the frequency ( b) and absolute numbers ( c) of memory-like CD4 + T cells in the indicated lymphoid organs of WT and Ube2n Treg-KO mice (8 weeks old), determined by flow cytometry. Data are representative of five experiments with three mice per group. ( a) Flow cytometric analysis of T cells derived from the indicated lymphoid organs of WT and Ube2n Treg-KO mice (8 weeks old), showing the percentage of naïve (CD44 loCD62L hi) and memory-like (CD44 hiCD62L lo) CD4 + T cells. Data are representative of 3–4 mice/genotype. ( d) H&E staining of various non-lymphoid tissue sections from 20 weeks old Ube2n Treg-KO and Ube2n +/+Foxp3 GFP-Cre (WT) mice. Data are representative of two independent experiments.

( c) Number of total cells from spleen, mesenteric lymph node (MLN) and peripheral lymph node (PLN) of age- and sex-matched Ube2n Treg-KO and Ube2n +/+Foxp3 GFP-Cre (WT) 10 week old mice presented as the mean±SD value. Results were from a total of three independent experiments (5–7 mice/genotype in each experiment). ( b) Bodyweight of Ube2n Treg-KO and Ube2n +/+Foxp3 GFP-Cre (WT) measured at the indicated times and presented as mean±SD. ( a) immunoblot of Ubc13 expression in CD4 + T cells and T reg cells sorted from Ube2n fl/flFoxp3 GFP-Cre (KO) and Ube2n +/+Foxp3 GFP-Cre (WT) mice.

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